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pp5 polyclonal rabbit cell signaling danvers  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pp5 polyclonal rabbit cell signaling danvers
    List of antibodies used
    Pp5 Polyclonal Rabbit Cell Signaling Danvers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp5 polyclonal rabbit cell signaling danvers/product/Cell Signaling Technology Inc
    Average 94 stars, based on 151 article reviews
    pp5 polyclonal rabbit cell signaling danvers - by Bioz Stars, 2026-05
    94/100 stars

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    1) Product Images from "Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain"

    Article Title: Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain

    Journal: Neurobiology of aging

    doi: 10.1016/j.neurobiolaging.2018.10.027

    List of antibodies used
    Figure Legend Snippet: List of antibodies used

    Techniques Used:

    Cortical proteins from the in 8-10 week old C57BL/6 mice that received either saline (Ctl) or midazolam 25 mg/kg i.p. and were sacrificed 30 min (Mid 0.5) or 24h (Mid 24) were separated by SDS-PAGE and protein levels of several protein phosphatases were determined using antibodies directed at (A) the demethylated protein phosphatase catalytic subunit (dm-PP2A-C), (B) the PP2A catalytic subunit (PP2A-C), (C) protein phosphatase 1 (PP1), (D) protein phosphatase 2B (PP2B), and (E) protein phosphatase 5 (PP5). Relative immunoreactive band intensities are expressed as a percent of saline control (Ctl) after appropriately normalizing for gel loading with β-actin or β-tubulin. For each condition, 2 representative immunoblot bands are displayed with Ctl (n = 6), 0.5h (n = 6), and 24h (n = 6). Data are expressed as mean ± SD. * denotes P < 0.05 vs. Ctl using ANOVA with Tukey’s post hoc test.
    Figure Legend Snippet: Cortical proteins from the in 8-10 week old C57BL/6 mice that received either saline (Ctl) or midazolam 25 mg/kg i.p. and were sacrificed 30 min (Mid 0.5) or 24h (Mid 24) were separated by SDS-PAGE and protein levels of several protein phosphatases were determined using antibodies directed at (A) the demethylated protein phosphatase catalytic subunit (dm-PP2A-C), (B) the PP2A catalytic subunit (PP2A-C), (C) protein phosphatase 1 (PP1), (D) protein phosphatase 2B (PP2B), and (E) protein phosphatase 5 (PP5). Relative immunoreactive band intensities are expressed as a percent of saline control (Ctl) after appropriately normalizing for gel loading with β-actin or β-tubulin. For each condition, 2 representative immunoblot bands are displayed with Ctl (n = 6), 0.5h (n = 6), and 24h (n = 6). Data are expressed as mean ± SD. * denotes P < 0.05 vs. Ctl using ANOVA with Tukey’s post hoc test.

    Techniques Used: Saline, SDS Page, Control, Western Blot



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    pp5 polyclonal rabbit cell signaling danvers  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc pp5 polyclonal rabbit cell signaling danvers
    List of antibodies used
    Pp5 Polyclonal Rabbit Cell Signaling Danvers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp5 polyclonal rabbit cell signaling danvers/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    pp5 polyclonal rabbit cell signaling danvers - by Bioz Stars, 2026-05
    94/100 stars
    		  Buy from Supplier

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    List of antibodies used

    Journal: Neurobiology of aging

    Article Title: Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain

    doi: 10.1016/j.neurobiolaging.2018.10.027

    Figure Lengend Snippet: List of antibodies used

    Article Snippet: A detailed summary of all of the antibodies used in these studies is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Phospho-Epitope Type Source Manufacturer Location Catalog # WB Dilution Tau Tau46 Total Tau Monoclonal Mouse Cell Signaling Danvers, MA 4019 1:2500 Anti-Human Tau A0024 Total Tau 243-441 Polyclonal Rabbit Dako Cytomation Carpinteria, CA A002401-2 1:10000 TG5 Total Tau 220-240 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT8 pSer202/pThr205 Monoclonal Mouse Thermo Scientific Waltham, MA MN1020 1:10000 CP13 pSer202 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 PHF-1 pSer396/pSer404 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT180 pThr231 Monoclonal Mouse Thermo Scientific Waltham, MA MN1040 1:1000 PS422 pSer422 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-764G 1:1000 PS262 pSer262 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-750G 1:1000 PS199 pSer199 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-734G 1:1000 AT270 pThr181 Monoclonal Mouse Thermo Scientific Waltham, MA MN1050 1:1000 12 E8 pSer262/pSer356 Monoclonal Mouse Pharmaceuticals Francisco, CA N/A 1:1000 Kinases GSK-3β Monoclonal Rabbit Cell Signaling Danvers, MA 9315 1:1000 Phospho-GSK-3 β (Ser9) Ser9 Polyclonal Rabbit Cell Signaling Danvers, MA 9336 1:1000 SAPK/JNK Monoclonal Rabbit Cell Signaling Danvers, MA 9258 1:1000 Phospho-SAPK/JNK Thr183/Tyr185 Monoclonal Rabbit Cell Signaling Danvers, MA 4671 1:1000 p44/42 MAPK Monoclonal Mouse Cell Signaling Danvers, MA 9107 1:2000 Phospho-p44/42 MAPK Thr202/Tyr204 Monoclonal Mouse Cell Signaling Danvers, MA 9106 1:2000 CaMKII Polyclonal Rabbit Cell Signaling Danvers, MA 3362 1:1000 Phospho-CaMKII Thr286 Polyclonal Rabbit Cell Signaling Danvers, MA 3361 1:1000 p35/25 Monoclonal Rabbit Cell Signaling Danvers, MA 2680 1:1000 Phosphatases PP2A-C (catalytic subunit) Monoclonal Mouse Sigma-Aldrich St. Louis, MO A5316 1:10000 demethylated PP2A-C Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 PP1 (E-9) Monoclonal Mouse Santa Cruz Dallas, TX sc-7482 1:1000 Pan-Calcineurin A (PP2B) Polyclonal Rabbit Cell Signaling Danvers, MA 2614 1:1000 PP5 Polyclonal Rabbit Cell Signaling Danvers, MA 2289 1:1000 Gel loading Controls β-actin Monoclonal Mouse Sigma-Aldrich St.Louis, MO A5316 1:10000 β3-tubulin Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 Open in a separate window List of antibodies used 2.3.

    Techniques:

    Cortical proteins from the in 8-10 week old C57BL/6 mice that received either saline (Ctl) or midazolam 25 mg/kg i.p. and were sacrificed 30 min (Mid 0.5) or 24h (Mid 24) were separated by SDS-PAGE and protein levels of several protein phosphatases were determined using antibodies directed at (A) the demethylated protein phosphatase catalytic subunit (dm-PP2A-C), (B) the PP2A catalytic subunit (PP2A-C), (C) protein phosphatase 1 (PP1), (D) protein phosphatase 2B (PP2B), and (E) protein phosphatase 5 (PP5). Relative immunoreactive band intensities are expressed as a percent of saline control (Ctl) after appropriately normalizing for gel loading with β-actin or β-tubulin. For each condition, 2 representative immunoblot bands are displayed with Ctl (n = 6), 0.5h (n = 6), and 24h (n = 6). Data are expressed as mean ± SD. * denotes P < 0.05 vs. Ctl using ANOVA with Tukey’s post hoc test.

    Journal: Neurobiology of aging

    Article Title: Administration of the benzodiazepine midazolam increases tau phosphorylation in the mouse brain

    doi: 10.1016/j.neurobiolaging.2018.10.027

    Figure Lengend Snippet: Cortical proteins from the in 8-10 week old C57BL/6 mice that received either saline (Ctl) or midazolam 25 mg/kg i.p. and were sacrificed 30 min (Mid 0.5) or 24h (Mid 24) were separated by SDS-PAGE and protein levels of several protein phosphatases were determined using antibodies directed at (A) the demethylated protein phosphatase catalytic subunit (dm-PP2A-C), (B) the PP2A catalytic subunit (PP2A-C), (C) protein phosphatase 1 (PP1), (D) protein phosphatase 2B (PP2B), and (E) protein phosphatase 5 (PP5). Relative immunoreactive band intensities are expressed as a percent of saline control (Ctl) after appropriately normalizing for gel loading with β-actin or β-tubulin. For each condition, 2 representative immunoblot bands are displayed with Ctl (n = 6), 0.5h (n = 6), and 24h (n = 6). Data are expressed as mean ± SD. * denotes P < 0.05 vs. Ctl using ANOVA with Tukey’s post hoc test.

    Article Snippet: A detailed summary of all of the antibodies used in these studies is shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Phospho-Epitope Type Source Manufacturer Location Catalog # WB Dilution Tau Tau46 Total Tau Monoclonal Mouse Cell Signaling Danvers, MA 4019 1:2500 Anti-Human Tau A0024 Total Tau 243-441 Polyclonal Rabbit Dako Cytomation Carpinteria, CA A002401-2 1:10000 TG5 Total Tau 220-240 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT8 pSer202/pThr205 Monoclonal Mouse Thermo Scientific Waltham, MA MN1020 1:10000 CP13 pSer202 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 PHF-1 pSer396/pSer404 Monoclonal Mouse Peter Davies Bronx, NY N/A 1:1000 AT180 pThr231 Monoclonal Mouse Thermo Scientific Waltham, MA MN1040 1:1000 PS422 pSer422 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-764G 1:1000 PS262 pSer262 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-750G 1:1000 PS199 pSer199 Polyclonal Rabbit Life Technologies Carlsbad, CA 44-734G 1:1000 AT270 pThr181 Monoclonal Mouse Thermo Scientific Waltham, MA MN1050 1:1000 12 E8 pSer262/pSer356 Monoclonal Mouse Pharmaceuticals Francisco, CA N/A 1:1000 Kinases GSK-3β Monoclonal Rabbit Cell Signaling Danvers, MA 9315 1:1000 Phospho-GSK-3 β (Ser9) Ser9 Polyclonal Rabbit Cell Signaling Danvers, MA 9336 1:1000 SAPK/JNK Monoclonal Rabbit Cell Signaling Danvers, MA 9258 1:1000 Phospho-SAPK/JNK Thr183/Tyr185 Monoclonal Rabbit Cell Signaling Danvers, MA 4671 1:1000 p44/42 MAPK Monoclonal Mouse Cell Signaling Danvers, MA 9107 1:2000 Phospho-p44/42 MAPK Thr202/Tyr204 Monoclonal Mouse Cell Signaling Danvers, MA 9106 1:2000 CaMKII Polyclonal Rabbit Cell Signaling Danvers, MA 3362 1:1000 Phospho-CaMKII Thr286 Polyclonal Rabbit Cell Signaling Danvers, MA 3361 1:1000 p35/25 Monoclonal Rabbit Cell Signaling Danvers, MA 2680 1:1000 Phosphatases PP2A-C (catalytic subunit) Monoclonal Mouse Sigma-Aldrich St. Louis, MO A5316 1:10000 demethylated PP2A-C Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 PP1 (E-9) Monoclonal Mouse Santa Cruz Dallas, TX sc-7482 1:1000 Pan-Calcineurin A (PP2B) Polyclonal Rabbit Cell Signaling Danvers, MA 2614 1:1000 PP5 Polyclonal Rabbit Cell Signaling Danvers, MA 2289 1:1000 Gel loading Controls β-actin Monoclonal Mouse Sigma-Aldrich St.Louis, MO A5316 1:10000 β3-tubulin Monoclonal Mouse Cell Signaling Danvers, MA 4466 1:1000 Open in a separate window List of antibodies used 2.3.

    Techniques: Saline, SDS Page, Control, Western Blot